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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB1 receptor density in cell membranes: an alternative to radioligand binding methods

Fig. 6

Radioligand binding assays in crude synaptosomes from rat brain cortex. A Representative saturation binding curves for [3H]SR141716A (0.01–10 nM) in the absence (empty circles) and in the presence of exogenously added 100 µM GTPγS (filled circles). B Representative saturation binding curve for [3H]-CP55940 (0.01–10 nM). Each point in curves corresponds to the mean ± SEM value of one independent experiment performed in triplicate. Mean values of the maximal number of sites (Bmax) and affinity (KD) averaged across three independent experiments are shown in Table 4

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Microbial Cell Factories

ISSN: 1475-2859

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