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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB1 receptor density in cell membranes: an alternative to radioligand binding methods

Fig. 4

Analysis of the stability over time and of the resistance to strong denaturalization of GST fusion constructs. Increasing amounts of affinity purified GST, GST-CB1414-442 and GST-CB1414-472 proteins stored at −80 °C for more than one year and subjected to harsh denaturing conditions were analyzed by SDS-PAGE and Coomassie blue staining

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