Fig. 3

Analysis of the integrity of the GST-fusion proteins GST-CB1_414–442 and GST-CB1_414–472. A Linear representation of GST, GST-CB1_414–442 and GST-CB1_414-472 proteins and their theoretical molecular masses. B Analysis by SDS-PAGE and Coomassie blue staining of whole-cell pellet lysates from Rosetta^™ (DE3) pLysS bacterial cultures induced by IPTG (3 h at 37 °C) for the expression of GST, GST-CB1_414–442 and GST-CB1_414–472 proteins. C Analysis by SDS-PAGE and Coomassie blue staining of GST, GST-CB1_414–442 and GST-CB1_414–472 proteins purified by affinity from the bacterial lysates shown in B. D Immunoblot of purified GST, GST-CB1_414–442 and GST-CB1_414–472 proteins using a rabbit polyclonal antibody raised against the 31 amino acid sequence at the C-terminus of the CB₁ receptor. Only the GST-CB1_414–472 construct carrying the antigen sequence was detected