Fig. 2

Agarose gel electrophoresis of PCR products and transformants. A Agarose gel electrophoresis analysis of PCR amplicons coding for CB1_414-472 and CB1_414–442 fragments of the cytosolic tail of the human CB₁ receptor with expected sizes of 191 and 101 bp, respectively. B BamHI/BsrGI restriction analysis of bacterial clones transformed with pCR-Blunt II-TOPO^™ vector containing DNA inserts coding for CB1_414–472 and CB1_414–442 polypeptides. The restriction maps are consistent with DNA fragments inserted in forward orientation. Expected sizes (bp) of restriction fragments were 3174/493/43 and 3174/403/43 for plasmid with DNA inserts coding for CB1_414–472 and CB1_414–442, respectively