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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus

Fig. 6

Expression of HA1 presented on the L. plantarum ZN-3 surface. a Schematic of pSIP401-SP1216-HA1-2578. A GS linker was inserted between DCpep and M cell-targeting peptide; b the protein of interest was identified on western blot. A relevant immunoreactive band was produced by the cell precipitation of strain pSIP401-HA1-ZN-3 (lane 1) and total cell supernatant of strain pSIP401-HA1-ZN-3(lane 3), but not strain pSIP401-ZN-3 (lanes 2 and 4). M: protein molecular weight marker; c Immunofluorescence microscopy pSIP401-ZN-3 (a1, a2, and a3) and pSIP401-HA1-ZN-3 (b1, b2, and b3) (magnification: 1000 ×)

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Microbial Cell Factories

ISSN: 1475-2859

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