Table 2 Genetic constructs used in this study
Construct name | Vector number | Antibiotic resistance in C. reinhardtii | Gene length with introns from start codon to stop (bp) | CDS (bp) | RBCS2 i1 copies | Protein length (aa) | Predicted molecular weight (kDA) | Accession No. (NCBI)/reference |
|---|---|---|---|---|---|---|---|---|
pOpt2_PsaD-AgBS-mVenus_Paro | i | Paromomycin | 4701 | 3357 | 7 | 1118 | 127.4 | This work |
pOpt2_PsaD-mCerulean3_Hyg | ii | Hygromycin B | 1395 | 921 | 1 | 306 | 50.0 | [6] |
pOpt2_PsaD-ERG20-mCerulean3_Hyg | iii | Hygromycin B | 2753 | 1989 | 3 | 662 | 74.8 | This work |
pOpt2_PsaD-mCerulean3-ERG20_Hyg* | iv | Hygromycin B | 2711 | 1947 | 3 | 648 | 73.2 | This work |
pOpt2_AgBS-mVenus_Paro | v | Paromomycin | 4605 | 3261 | 7 | 1086 | 124.2 | [8] |
pOpt2_AgBS-mVenus-ERG20_Paro* | vi | Paromomycin | 6014 | 4380 | 9 | 1459 | 166.5 | This work |
pOpt2_AgBS-mVenus-CrFPPS_Paro* | vii | Paromomycin | 6084 | 4305 | 10 | 1434 | 163.0 | This work |
pOpt2_AgBS-mVenus-CrSQSTMD_Paro | viii | Paromomycin | 4839 | 3495 | 7 | 1164 | 132.8 | This work |
pOpt2_PsaD-AgBS-mVenus-CrFPPS_Paro* | ix | Paromomycin | 6192 | 4413 | 10 | 1470 | 166.8 | This work |
pOpt2_PsaD-AgBS-mVenus-ERG20_Paro* | x | Paromomycin | 6014 | 4380 | 9 | 1459 | 166.5 | This work |
pOpt2_PsaD-mRuby2_Ble | xi | Zeocin | 1389 | 915 | 1 | 304 | 33.9 | This work |
pOpt2_PsaD-AgBS-mRuby2_Ble | xii | Zeocin | 4707 | 3363 | 7 | 1120 | 127.5 | This work |
pOpt2_PsaD-aadA2_MinS | xiii | Spectinomycin | 1467 | 993 | 1 | 330 | 36.7 | [6] |
pOpt2_PsaD-AgBS-aadA2_MinS | xiv | Spectinomycin | 4785 | 3441 | 7 | 1146 | 130.3 | This work |
pOpt2_PsaD-AgBS-mCerulean3-ERG20_Hyg* | xv | Hygromycin B | 6029 | 4395 | 9 | 1464 | 166.8 | This work |
pOpt2_PsaD-AgBS-mRuby2-ERG20_Ble* | xvi | Zeocin | 6023 | 4389 | 9 | 1462 | 166.6 | This work |
pOpt2_PsaD-AgBS-aadA2-ERG20_MinS* | xvii | Spectinomycin | 6101 | 4467 | 9 | 1488 | 169.5 | This work |
pOpt2_mCerulean3_Hyg | xviii | Hygromycin B | 1287 | 813 | 1 | 270 | 30.4 | [8] |
pOpt2_CrFPPS-mCerulean3_Hyg | xix | Hygromycin B | 2820 | 1911 | 4 | 636 | 71.3 | This work |
pOpt2_mCerulean3-CrFPPS_Hyg* | xx | Hygromycin B | 2778 | 1869 | 4 | 622 | 69.6 | This work |
UniProt ID | ||||||||
AgBs alone | O81086 | 3318 | 2448 | 6 | 816 | 93.6 | MG052654 [8] | |
ERG20 alone | P08524 | 1346 | 1056 | 2 | 352 | 40.5 | KX097889 [7] | |
CrFPPS alone | A8IX41 | 1515 | 1080 | 3 | 360 | 40.5 | This work |