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Table 6 qPCR and RNA-seq results of genes from carbon transport systems and central metabolism

From: Glucose consumption rate-dependent transcriptome profiling of Escherichia coli provides insight on performance as microbial factories

 

Gene

RT-qPCRa

RNA-seq

WG

WGM

WGMC

WHIC

WG

WGM

WGMC

WHIC

AVG

SD

AVG

SD

AVG

SD

AVG

SD

Transport systems

galP

1.19

0.01

1.63

0.29

1.48

0.18

1.59

0.37

− 0.43

0.18

0.58

1.40

manX

5.88

1.25

ND

ND

ND

ND

1.19

0

2.11

ND

ND

1.80

nagE

4.38

0.54

43.34

4.43

53.34

3.83

30.95

11.49

2.08

5.10

5.04

5.96

lamB

22.73

4.61

8.42

0.18

7.27

0.45

1.64

0.2

3.89

3.69

3.86

2.25

ompC

0.25

0.11

0.73

0.06

0.91

0.27

0.96

0.14

− 1.00

0.46

0.5

0.55

ompF

2.93

0.07

0.58

0

0.56

0.08

0.14

0.02

0.97

− 0.61

− 0.26

− 1.55

mglB

29.11

10.46

4.55

0.62

ND

ND

ND

ND

4.46

1.88

ND

ND

 

Gene

RT-qPCR

RNA-seq

WG

WGM

WGMC

WHIC

WG

WGM

WGMC

WHIC

AVG

SD

AVG

SD

AVG

SD

AVG

SD

TCA

gltA

3.2

1

0.65

0.02

0.93

0.05

1.39

0.24

1.35

− 0.10

0.20

0.80

 

Gene

RT-qPCR

RNA-seq

WG

WGM

WGMC

WHIC

WG

WGM

WGMC

WHIC

AVG

SD

AVG

SD

AVG

SD

AVG

SD

    

Acetate

acs

27.58

4.97

3.42

0.39

3.83

0.32

3.13

0.44

4.12

1.74

1.84

2.61

poxB

0.44

0.02

1.78

0.23

1.84

0.37

1.55

0.2

0.38

1.16

1.21

1.98

ppsA

1.82

0.37

0.41

0.06

1.89

0.53

3.72

0.4

0.98

− 0.39

0.48

2.37

  1. aValues represent the logarithm of fold change when comparing gene expression values from genes of each strain against E. coli W3110. Dispersion values are not presented from RNA-seq results but can be consulted in the supplementary files
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