Table 1 Strains used in this study
From: Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris
Strain | Genotype | Reference |
|---|---|---|
DH5α | F−, φ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(\(r_{k^{-}}\), \(m_{k^{+}}\)), phoA, supE44, λ−, thi-1, gyrA96, relA1 | Takara |
GS115 | Mut+, his4−, AOX1, AOX2 | Lab stock |
GS115-RAD52 | GS115, HIS4::PGAP -PpRAD52-TAOX1 | [17] |
GS115-RAD52-mph1Δ | GS115, HIS4::PGAP -PpRAD52-TAOX1, mph1Δ | [17] |
GS115-RAD52-srs2Δ | GS115, HIS4::PGAP -PpRAD52-TAOX1, srs2Δ | This study |
GS115-RAD52-mph1Δ-srs2Δ | GS115, HIS4::PGAP -PpRAD52-TAOX1, mph1Δ, srs2Δ | This study |