Fig. 6
From: Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris
The effect of CAS9-MRE11 on the integration of multi-fragments. A The fatty alcohol biosynthetic pathway was integrated into FAA1 locus. This pathway was divided to the three parts with overlapping 500 bp at the promoter or terminator regions and 1000 bp at homologous arms. B Integration efficiency of CAS9-MRE11 on multi-fragment in the background strains RAD52, RAD52-mph1Δ, RAD52-srs2Δ, and RAD52-mph1Δ-srs2Δ. 500 ng of each donor DNA was used for evaluating integration efficiency. Twenty clones from each plate were picked for colony PCR analysis. Data are presented as mean ± s.d. with three biologically independent samples. C Schematic diagram of Srs2 protein inhibiting HR process by interfering with Rad51