Fig. 5
From: Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris
The effect of MRE11-CAS9 and overexpression of RAD52 on simultaneous deletion of multiple genes. A Simultaneously deleting two genes FAA2 and HFD1. B Simultaneously deleting three genes FAA2, HFD1, and POX1. Deletion of multiple genes was carried out in the RAD52 overexpression strain GS115-RAD52, which was transformed with 500 ng each donor DNA, and plasmids expressing gRNA and CAS9/CAS9-MRE11. Twenty clones from each plate were picked for colony PCR analysis. Data are presented as mean ± s.d. with three biologically independent samples