Fig. 2
From: Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris
Schematic representation of the fusion expression of Cas9 and exonucleases. A bidirectional promoter PHTX1 was used to express the CAS9 gene, exonuclease genes and the gRNA. The gRNA was flanked by hammerhead (HH) and hepatitis delta virus (HDV) ribozymes. These two ribozymes were transcribed and auto-catalytically cleaved themselves to generate a mature gRNA. Exonucleases were fused to N-terminus (A) or C-terminus (B) of Cas9 with a linker (GGGGS)3, respectively