Fig. 1From: Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastorisHomologous recombination process mediated by CRISPR/Cas9. The fusion of an exonuclease and Cas9 is advantageous in promoting the exonuclease to bind with DSBs, which subsequently initiates resection of the DSBs toward HR and inhibits NHEJ pathway. The genes marked as red were overexpressed or deleted for enhancing HR efficiencyBack to article page