Glucose transport engineering allows mimicking fed-batch performance in batch mode and selection of superior producer strains

Table 3 Specific growth rates and final GFP concentration in batch cultures with 20 g L⁻¹ glucose. ND: Not detected by the method used; µ: specific growth rate; SGF: specific GFP fluorescence. Subscripts 1 and 2 refer to the first and second growth phase, respectively

Strain	µ₁ [h⁻¹]	SGF₁ [–]	µ₂ [h⁻¹]	SGF₂ [–]	Final GFP conc. [mg L⁻¹]
W3110	0.70 ± 0.03	1.00 ± 0.08	0.12 ± 0.01	1.24 ± 0.09	1.5 ± 0.0
WG	0.52 ± 0.03	0.20 ± 0.01	0.24 ± 0.02	0.24 ± 0.01	ND
WGB	0.43 ± 0.03	0.20 ± 0.00	0.26 ± 0.01	0.29 ± 0.01	ND
WGE	0.42 ± 0.02	0.21 ± 0.01	0.23 ± 0.01	0.31 ± 0.01	ND
WGM	0.66 ± 0.04	0.53 ± 0.02	0.04 ± 0.00	1.28 ± 0.08	1.91 ± 0.40
WGMB	0.61 ± 0.27	1.00 ± 0.05	0.03 ± 0.00	2.73 ± 0.23	1.35 ± 0.80
WGME	0.59 ± 0.06	0.28 ± 0.01	0.04 ± 0.00	0.90 ± 0.01	0.82 ± 0.21
WGMP	0.46 ± 0.03	0.66 ± 0.03	0.03 ± 0.01	0.95 ± 0.10	0.53 ± 0.04
WGMX	0.66 ± 0.02	0.13 ± 0.01	0.05 ± 0.00	0.63 ± 0.05	0.48 ± 0.04
WGP	0.43 ± 0.03	0.77 ± 0.06	0.31 ± 0.01	0.35 ± 0.01	ND
WGX	0.94 ± 0.02	0.57 ± 0.05	0.07 ± 0.01	1.07 ± 0.09	0.82 ± 0.04
WHI	0.51 ± 0.07	0.05 ± 0.01	0.04 ± 0.00	0.22 ± 0.01	ND
WHIC	0.46 ± 0.01	1.73 ± 0.01	0.06 ± 0.00	6.05 ± 0.02	5.15 ± 0.76

ISSN: 1475-2859