Fig. 5

GFP expression performance of the wild type and mutant strains cultures in semi-defined medium supplemented with 10 g/L glucose and 4 g L⁻¹ yeast extract (A, B) or 20 g L⁻¹ glucose and 8 g L⁻¹ yeast extract (C, D). Top panel: maximum GFP fluorescence; bottom panel: specific GFP fluorescence. Values were calculated over the slow-growth phase. Culture conditions: 48 well FlowerPlates. V_L = 800 µL, n = 1500 rpm, d₀ = 3 mm. Note that the scales are not identical. Vertical lines indicate the standard deviation between triplicate experiments