Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

Table 1 N-terminal extensions of the RBD (amu)

N-terminal signal peptide extensions	Theoretical mass of extension	Theoretical mass of RBD + extension after PNGase treatment	Measured mass	Error
(none)	N/A	22,582.41	22,582.43	0.02
EEGVSLEKR	1028.13	23,610.54	23,610.36	−0.18
AKEEGVSLEKR	1227.38	23,809.79	23,809.72	−0.07
SIAAKEEGVSLEKR	1498.69	24,081.11	24,079.86	−1.25
FINTTIASIAAKEEGVSLEKR	2259.58	24,842.00	24,842.89	0.89
SNSTNNGLLFINTTIASIAAKEEGVSLEKR	3160.53	25,744.92	25,745.42	0.50
APVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR	6938.62	29,524.00	29,524.7	0.70

ISSN: 1475-2859