Fig. 4
Single amino acids eliminate N-terminal extension. A Secreted titer and cell density of strains producing RBD variants with different N-terminal sequences. Cells were cultivated at 3Â mL scale for 1Â day of outgrowth and 1Â day of production. Bars represent the average of three independent cultures. B Reduced SDS-PAGE of purified RBD variants. C Reduced SDS-PAGE of purified RBD variants after treatment with PNGase