Fig. 2
Addition of residues to the N-terminus eliminates N-terminal extension. A Reduced SDS-PAGE of unpurified supernatant and purified variants of the RBD. B Intact LCMS of purified RBD variants. C Secreted titer and cell density of strains producing RBD variants with different N-terminal sequences. Cells were cultivated at 3Â mL scale for 1Â day of outgrowth and 1Â day of production. Bars represent the average of three independent cultures