Fig. 1

The effect of N-glycosylation on molecular weight, autocatalytic cleavage activity, thermal stability of prochymosin and the secretion in P. pastoris GS115. A Mutant site of N-glycosylation in the propeptide of prochymosin, conservated N-glycosylated sequences were indicated by underlines; B molecular weight and de-glycosylation analysis of chy and chy34 by Western-blot; C Determination of the secretion of chy and chy34 by ELISA; D The mRNA levels of chy and chy34 in host cells determined by qRT-PCR; E Analysis of autocatalytic cleavage of the propeptide by confirming the milk-clotting acidity, The control was the supernatant of GS115 culture carried the pPIC9K vector; F Thermostability of chy and chy34 when stored at different temperatures for 8 h prior to enzymatic activity assay. Error bars indicate the standard deviation of tree independent experiments, student’s t-test was used to compare difference between two groups, *P < 0.05