Table 1 Bacterial strains and plasmids used in this study

Strain

APEC χ7122

Wild type strain

[29]

APEC χ7122 pgl⁺

pgl integrant, kan^r

[14]

APEC χ7122 pgl⁺ ΔwecA::cat

pgl integrant with wecA deleted and replaced with cat, kan^r, cm^r

This study

Plasmid

pFPV25.1-G-NetB(10)

Plasmid stably maintained in APEC that expresses NetB with 10 PglB target sites under the control of a constitutive rpsM promoter, amp^r

[14]

pFPV25.1-FlpA-10GT

Plasmid stably maintained in APEC that expresses FlpA with 10 PglB target sites under the control of a constitutive rpsM promoter, amp^r

This study

pBADλred

λRed recombineering plasmid, amp^r

[26]