Fig. 2

Graphs showing FlpA glycoconjugate staining at various temperatures and permeability states. Percentage of total bacterial cells positive for anti-6xHis-tag staining (yellow), lectin staining (purple) and anti-6xHis-tag + lectin staining (black), empty bars = non-permeabilized, hatched bars = permeabilized, at (a) 28 °C, (b) 37 °C and (c) 42 °C. A APEC χ7122 pgl⁺  + pFPV25.1-flpA-10GT, B APEC χ7122 pgl⁺ ΔwecA::cat + pFPV25.1-flpA-10GT, (C) APEC χ7122 pgl⁺, D APEC χ7122, E APEC χ7122 pgl⁺  + pFPV25.1-flpA-10GT with permeabilization, F APEC χ7122 pgl⁺ ΔwecA::cat + pFPV25.1-flpA-10GT with permeabilization, G APEC χ7122 pgl⁺ with permeabilization, H APEC χ7122 with permeabilization. Statistically significant differences between samples (within unpermeabilized and permeabilized groups) are annotated with letters indicating the group(s) for which significance is observed, statistically significant differences between groups are annotated with lines. Two-tailed, unpaired T-test. P < 0.05. Expression for each sample within each group is compared. Expression between groups for only the equivalent sample is compared. N = 4 biological repeats