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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Modification of avian pathogenic Escherichia coli χ7122 lipopolysaccharide increases accessibility to glycoconjugate antigens

Fig. 1

Confocal microscopy images of APEC χ7122 pgl+ expressing glycosylatable FlpA. Bacteria were stained with lectin SBA from Glycine max (soybean) Alexa Fluor™ 647 conjugate (magenta), 6xHis-tag monoclonal antibody (HIS.H8) Alexa Fluor 488 (yellow) and Hoechst nuclear stain (cyan). A APEC χ7122 pgl+  + pFPV25.1-flpA-10GT, B APEC χ7122 pgl+  + pFPV25.1-flpA-10GT with permeabilization, C APEC χ7122 pgl+ ΔwecA::cat + pFPV25.1-flpA-10GT, D APEC χ7122 pgl+ ΔwecA::cat + pFPV25.1-flpA-10GT with permeabilization, E APEC χ7122 pgl+, F APEC χ7122 with permeabilization. Images B, D and F lack Hoechst nuclear stain due to permeabilization requiring DNase treatment. Scale bar = 10 µm

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Microbial Cell Factories

ISSN: 1475-2859

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