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Table 1 Product of conversion, UPLC–DAD, HR-QTOF ESI/MS analysis, and UV maxima of substrates in vivo reaction using RgF8H

From: Novel flavonoid C-8 hydroxylase from Rhodotorula glutinis: identification, characterization and substrate scope

Substrates

Products

Name

UPLC (tR) [min]

Mass

UV maxima [nm]

Name

Conversion (%)

UPLC (tR) [min]

Mass

UV maxima [nm]

[M+H]+ m/z+

[M−H]− m/z−

[M+H]+ m/z+

[M−H]− m/z−

Naringenin

3.271

273

271

288.90

8-hydroxynaringenin

63.84

2.768

289

287

293.52

Eriodictyol

2.993

289

287

287.98

8-hydroxyeriodictyol

64.94

2.454

305

303

291.78

Pinocembrin

3.623

257

255

289.13

8-hydroxypinocembrin

61.61

3.121

273

271

294.62

Hesperetin

3.212

303

301

287.43

8-hydroxyhesperetin

30.64

2.761

319

317

292.35

Luteolin

3.245

287

285

252.66, 346.00

8-hydroxyluteolin

73.09

2.781

303

301

278.55, 338.04

Apigenin

3.550

271

269

267.38, 334.78

8-hydroxyapigenin

20.02

3.039

289

287

279.79, 303.08

Chrysin

3.792

255

253

267.31

8-hydroxychrysin

8.92

3.281

271

269

280.01

Diosmetin

3.409

301

299

250.98, 343.60

8-hydroxydiosmetin

7.38

2.955

317

315

279.77, 336.07

  1. The percentage of conversion was calculated based on the decrease of substrates, due to the instability of the obtained hydroxyflavonoid derivatives
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Microbial Cell Factories

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