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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Novel flavonoid C-8 hydroxylase from Rhodotorula glutinis: identification, characterization and substrate scope

Fig. 5

Phylogenetic analysis based on C-6, C-8, and Riske-type hydroxylase sequences. Protein sequences downloaded from the National Center for Biotechnology Information (NCBI) were aligned using Clustal Omega software (https://www.ebi.ac.uk/Tools/msa/clustalo). The phylogenetic tree was constructed using iTOL software (https://itol.embl.de/). Bootstrap values are given next to each branch. The line length indicates the evolutionary distance calculated by the Poisson correction method, and the branch labels are the protein names. Labels used: blue colour—Riske enzymes, pink colour—C-6 hydroxylases, yellow colour—C-8 hydroxylases. The nucleotide sequences of the proteins used in this study are available in the Additional file 1: Table S3

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