Fig. 3

Time-course of improved fed batch fermentations. In all the fermentations the growth phase was extended (3✕). In the production a total of 200 g of glycerol was supplied through an initial pulse and followed by continuous feeding at 1.75 g/L/h. A Pulse of 114.5 g/L glycerol, 32.5 °C. B Pulse of 200 g/L glycerol, 36 °C. C Pulse of 200 g/L glycerol, 38 °C. Optimal pH and DOT values were maintained. The experiments were carried out in triplicate. Representative time-courses are reported herein