Fig. 2

Time-course of fed batch fermentations, carried out under the optimal conditions (glycerol concentration = 114.5 g/L, temperature = 32.5 °C, pH = 5.875 and DOT = 31.2%). A Fed batch process utilized for model validation. B Fed batch process with extended growth phase (3✕) and with application of repeated glycerol pulses. The experiments were carried out in triplicate. Representative time-courses are reported herein