Table 1 Summary of genome editing efficiency and plasmid curing efficiency of CRISPR-Cas9 system
Plasmid | CFU(counts/mL) | Editing efficiency | Curing efficiency |
|---|---|---|---|
pGE-KOerm | 87 ± 7 | 32/32 (100%) | 46/48 (95.8%) |
pGE-KOamyE | 42 ± 2 | 32/32 (100%) | 44/48 (91.6%) |
pGE-PMspo0A | 660 ± 42 | 660/660 (100%) | 47/48 (97.9%) |
pGE-Iegfp | 94 ± 4 | 30 ± 1/32 (93.8% ± 3.1%) | 46/48 (95.8%) |