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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis

Fig. 2

Enhancing transformation efficiency through PAMPL method. a Different transformation methods for B. subtilis SCK6. PCR inevitably introduce unnecessary mutations and it is not suitable for large-sized plasmid. POE method requires a high concentration of monomeric plasmid to ensure the formation of multimeric plasmid. Direct transformation of monomeric plasmids into B. subtilis SCK6 is inefficient. The PAMPL method we exploited could improve the transformation efficiency of B. subtilis SCK6 by using PEG4000. b Gel electrophoresis of different types of CRISPR-Cas9 plasmid pGE-KOerm. M: 1 kb Marker; 1. monomeric plasmid; 2. linearized plasmid; 3. multimeric plasmid. c Transformation efficiency of different types of plasmids in B. subtilis SCK6. *Represent p < 0.05, ***represent p < 0.001

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Microbial Cell Factories

ISSN: 1475-2859

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