Strain specific properties of Escherichia coli can prevent non-canonical amino acid misincorporation caused by scale-related process heterogeneities

Table 1 MS results for the percentage of Fab fragments found with norleucine

Fragment	H < oFTN2 > Scale-down 1 Norleucine [%]	H < oFTN2 > Scale-down 2 Norleucine [%]	Average Norleucine [%]	Deviation of single experiments to average [%]
HC 1	18.44	14.53	16.49	± 1.96
HC 2	13.69	8.50	11.10	± 2.60
HC 3	10.66	8.65	9.66	± 1.01
HC 4	14.35	11.25	12.8	± 1.55
LC 1	20.83	17.62	19.23	± 1.61
Average	15.59	12.11	13.85	± 1.74

The results for the biological duplicates of H < oFTN2 > cultivated with the scale-down setup at 29 h after feed start are given. Four fragments resulted from the Fab heavy chain (HC) and one from Fab light chain (LC). The column ‘Average Norleucine’ represents the average of the biological duplicates, the column ‘Deviation of single experiments to average’ gives the deviation of the biological duplicates to the average

ISSN: 1475-2859