Table 1 Bacterial strains and plasmids used in this study
Strain | Genotype or description | Source |
|---|---|---|
W3110 | F− λ− IN(rrnD-rrnE) rph-1 ∆fnr | Laboratory collection |
68A8 | ΔfhuA ΔphoA ilvG2096 (IlvG+, Valr) ΔmanA ΔtrxA14::kan | Laboratory collection |
JW0871-1 | F−, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ−, ΔtrxB786::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Coli Genetic Stock Center (CGSC) |
JW3467-1 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ−, Δgor756::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | CGSC |
JW2861-1 | F-, c(araD-araB)567, ΔlacZ4787(::rrnB-3), λ−, ΔdsbC744::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | CGSC |
64B4 | W3110 ∆fhuA ∆phoA ilvG2096 (IlvG+; Valr) ∆manA ∆prc spr43H1 ∆degP lacIq ∆ompT | Laboratory collection |
68B1 | 64B4 ΔtrxA14::kan | This study |
68A6 | 64B4 ΔtrxB786::kan | This study |
68A7 | 64B4 Δgor756::kan | This study |
68B9 | 64B4 Δgor756 | This study |
68C1 | 64B4 ΔtrxA14::kan Δgor756 | This study |
68D3 | 64B4 ΔdsbC744::kan | This study |
68D5 | 64B4 ΔdsbC744 | This study |
Plasmid | ||
 pmAb1 | pBR322 plasmid coding for mAb1 and also overexpressing DsbA and DsbC | Laboratory collection |
 pRM1 | pmAb1 without the dsbC gene | This study |