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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Enzymatic basis of the Fc-selective intra-chain disulfide reduction and free thiol content variability in an antibody produced in Escherichia coli

Fig. 8

Effect of Fc glycosylation status on free thiol formation. a RP-HPLC profiles of glycosylated and de-glycosylated mAb2 MSS pools derived from homogenate held for 16 h b Homogenate hold-induced free thiol levels in fully deglycosylated mAb2 or mAb2 with enzymatically trimmed glycans. Results shown are for n = 1 sample

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