Fig. 8From: Enzymatic basis of the Fc-selective intra-chain disulfide reduction and free thiol content variability in an antibody produced in Escherichia coliEffect of Fc glycosylation status on free thiol formation. a RP-HPLC profiles of glycosylated and de-glycosylated mAb2 MSS pools derived from homogenate held for 16 h b Homogenate hold-induced free thiol levels in fully deglycosylated mAb2 or mAb2 with enzymatically trimmed glycans. Results shown are for n = 1 sampleBack to article page