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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Enzymatic basis of the Fc-selective intra-chain disulfide reduction and free thiol content variability in an antibody produced in Escherichia coli

Fig. 7

DsbC is responsible for disulfide bond reduction in mAb1 during blank host homogenate hold. A blank host fermentation was carried out using the same production host and process, but the plasmid did not contain genes for expression of mAb1 light and heavy chains and dsbC. a Dose dependent increase in free thiol in presence of DsbC. Note that “-DsbC” indicates that no DsbC was spiked. A blank host homogenate contains DsbC due to chromosomal expression, b RP-HPLC profiles of mAb1 MSS pools derived from homogenate held for 16 h in presence of DsbC. Results shown are for n = 1 sample

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Microbial Cell Factories

ISSN: 1475-2859

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