Fig. 6

mAb1 fermentations using mutants lacking enzymes in thiol redox pathways. a Thiol-redox pathways in E. coli cytoplasm (selected mutants shown in bold). b Growth, c Titer (product titer in black bars and DsbC titer (control case) in grey bar) d Free thiol content during homogenate hold. The ΔtrxA, ΔtrxB, Δgor, and ΔtrxA Δgor mutants contain pmAb1 plasmid coding for mAb1 and also overexpressing DsbA and DsbC. The ΔdsbC mutant contains pRM1 plasmid (pmAb1 without the dsbC gene). For determining free thiol formation in homogenate derived from ΔdsbC mutant fermentation, mAb1 was spiked at 4 mg/mL final concentration. OD₅₅₀ results shown for control and ΔdsbC cases are average of n = 3 runs. Titer results shown for control and ΔdsbC cases are average of n = 3 runs and 1 s.d. For ΔtrxB, ΔtrxA, Δgor, and ΔtrxA Δgor cases, OD₅₅₀ and titer results shown are for n = 1 run. RP-HPLC assay results are for n = 1 sample