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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enzymatic basis of the Fc-selective intra-chain disulfide reduction and free thiol content variability in an antibody produced in Escherichia coli

Fig. 3

Effect of process parameters on total free thiol content in mAb1. a Clearance of mAb1 free thiols across 4 chromatography steps. Example of materials with low (Low) and high (High) total free thiol content are shown. b Correlation of the total free thiol content of MSS pools and corresponding CUFDF pools. c Effect of whole cell broth hold at the end of production culture step on total free thiol content. d Effect of temperature and presence of the flocculant (FL) on total free thiol content during the homogenate hold step. Results shown are for n = 1 sample

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Microbial Cell Factories

ISSN: 1475-2859

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