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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Enzymatic basis of the Fc-selective intra-chain disulfide reduction and free thiol content variability in an antibody produced in Escherichia coli

Fig. 2

Free thiol content in mAb1. a Variability of the total free thiol content of the mAb1 DS batches produced at manufacturing-scale (MSS, MabSelectSure; AEX, Anion exchange, CEX, cation exchange). b Example chromatograms of mAb1 with the “Low” and “High” total free thiol content analyzed by RP-HPLC with the free thiol derivatization and UV detection (CUFDF, conditioned UFDF). c Site-specific levels of free thiols in mAb1 DS materials during development with the varying total free thiol content. Results shown are for n = 1 sample

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Microbial Cell Factories

ISSN: 1475-2859

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