Fig. 1

Two different natural biosynthesis pathways of ergothioneine (A). A phylogenetic tree with schematic diagram of functional domains contains putative Egt1 (B) or Egt2 (C) homologous (boxes represented the predicted domains and labeled with corresponding superfamily names). A schematic diagram of genes structure (boxes represented exon and the black line between boxes represented intron) of NcEgt1/2 and CmEgt1/2 (D). Sequence alignment of putative Egt1 (E) or Egt2 (F) homologous in edible mushrooms (predicted key residues for binding were labeled)