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Table 2 Activities of the enzymes xylose reductase (XR), xylitol dehydrogenase (XDH), alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC) in the tested O. polymorpha strains with the HXS1 and AZF1 genes deletion and overexpression. Activities were measured on 72 h of fermentation on xylose at 37 °C

From: The role of hexose transporter-like sensor hxs1 and transcription activator involved in carbohydrate sensing azf1 in xylose and glucose fermentation in the thermotolerant yeast Ogataea polymorpha

Strain

Activity U/mg of protein

XR

XDH

ADH

PDC

NCYC 495 WT

0.99 ± 0.05

0.62 ± 0.03

0.97 ± 0.05

0.33 ± 0.02

WT/Δazf1

0.98 ± 0.04

0.64 ± 0.03

0.82 ± 0.03

0.43 ± 0.02

WT/AZF1

0.89 ± 0.04

0.50 ± 0.02

0.76 ± 0.03

0.53 ± 0.02

WT/Δhxs1

0.82 ± 0.04

0.56 ± 0.03

0.80 ± 0.04

0.52 ± 0.02

WT/HXS1

0.85 ± 0.04

0.67 ± 0.03

0.81 ± 0.03

0.37 ± 0.02

BEP/Δcat8

0.84 ± 0.04

1.05 ± 0.05

1.07 ± 0.05

0.69 ± 0.03

BEP/Δcat8/AZF1

0.87 ± 0.04

1.42 ± 0.06

0.70 ± 0.03

0.92 ± 0.05

BEP/Δcat8/HXS1

0.87 ± 0.04

1.47 ± 0.07

1.06 ± 0.05

0.83 ± 0.04