The role of hexose transporter-like sensor hxs1 and transcription activator involved in carbohydrate sensing azf1 in xylose and glucose fermentation in the thermotolerant yeast Ogataea polymorpha

Table 1 Main parameters of xylose fermentation at 37 °C by the tested O. polymorpha strains with HXS1 and AZF1 genes deletion and overexpression

Strain	Ethanol (g/L)	Ethanol yield (mg/g of consumed xylose)	Ethanol specific production rate (mg/g biomass/h)	Ethanol productivity (mg/L/h)
WT^b	0.49 ± 0.03	15 ± 2	4 ± 1	7 ± 1
WT/LEU^b	0.56 ± 0.07	19 ± 2	5 ± 1	8 ± 1
Ins^b	0.02 ± 0.00	1 ± 0	0 ± 0	0 ± 0
WT/Δhxs1^b	0.03 ± 0.00	2 ± 0	0 ± 0	0 ± 0
WT/Δhxs1/LEU^b	0.01 ± 0.00	0 ± 0	0 ± 0	0 ± 0
WT/Δhxs/LEU/HXS1^b	0.93 ± 0.06	20 ± 2	7 ± 1	13 ± 2
WT/LEU/HXS1^b	0.83 ± 0.07	25 ± 4	8 ± 1	12 ± 3
WT/AZF1^a	1.20 ± 0.05	41 ± 8	10 ± 3	25 ± 2
WT/∆azf1^b	0.33 ± 0.03	20 ± 4	3 ± 1	4 ± 1
BEP/∆cat8^b	9.10 ± 0.10	154 ± 20	49 ± 11	126 ± 22
BEP/∆cat8/AZF1^c	11.02 ± 0.11	354 ± 31	40 ± 19	114 ± 19
BEP/∆cat8/HXS1^b	13.04 ± 0.12	352 ± 29	63 ± 17	181 ± 27

^aData of ethanol yield (mg/g of consumed xylose), ethanol specific production rate (mg/g biomass/h), ethanol productivity (mg/L/h) and ethanol concentration (g/L) are represented on YNB medium supplemented with 10% of xylose on 48 h of fermentation
^b72 h of fermentation
^c96 h of fermentation

ISSN: 1475-2859