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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Human growth hormone inclusion bodies present native-like secondary and tertiary structures which can be preserved by mild solubilization for refolding

Fig. 1

Alkaline pH and high pressure promote solubilization of hGH-IBs. A SDS-PAGE of hGH-IBs (reducing condition); B real-time determination of LS of a suspension of hGH-IBs subjected to 2.4 kbar at pH 10.0; C determination of LS of suspensions of hGH-IBs that had been subjected to 2.4 kbar or maintained at atmospheric pressure for 90 min at 20 °C. This experiment was performed in duplicate (1 bar) or triplicate (2.4 kbar); D SDS-PAGE of supernatants of samples subjected to 1 bar or to 2.4 kbar (reducing conditions); E scanning electron micrography of hGH-IBs and of the remaining insoluble fraction of a sample that was subjected to 2.4 kbar for 90 min at pH 9.0

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Microbial Cell Factories

ISSN: 1475-2859

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