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Table 1 Quantification of overall illudin M yields after each unit operation during DSP

From: Optimization of the production process for the anticancer lead compound illudin M: downstream processing

Unit operation

Volume after UO

Titer

Total yield

Yield

(UO)

(L)

(mg mL−1)

(g)

(%)

Centrifugation

 ~ 3.000

0.575

1.725

100

Antifoam removal

 ~ 3.000

0.575

1.725

100

Depth filtration

 ~ 3.000

0.575

1.725

100

Adsorption

 ~ 0.785

1.860

1.460

85

Crystallization

n.a

n.a

1.328

77

  1. In three independent experiments starting from cell free supernatant, final yields of crystals between 72 and 79% could be achieved
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Microbial Cell Factories

ISSN: 1475-2859

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