Fig. 9

UV signal at 325 nm and illudin M concentration during isocratic elution with different methanol mixtures. The black curve illustrates the UV signal at 325 nm while eluting illudin M from XAD16N with isocratic steps. The blue squares illustrate the illudin M concentrations in samples taken over the run. The colored blocks indicate the methanol concentration and the duration of the isocratic wash and elution steps