Fig. 5

Illudin M concentration indicated in weight percent (wt%) in the dry mass of eluates from two subsequent extraction steps. Blue dots illustrate the wt% of illudin M in the extract from the first elution with increasing methanol concentrations and the respective regression curve. Red squares illustrate the wt% of illudin M in the extract after the second elution with 100% methanol and the respective regression curve. Outliers of the second extraction are illustrated as black squares and may result from measurement errors due to the low weight of the samples. Both data sets were generated with triplicate samples of each eluted fraction