Fig. 10
UV signal and illudin M concentration measured while overloading a packed column. A XAD16N column was loaded with 10 L of clarified supernatant (~ 700 mg L−1 illudin M). The scatter plot illustrates the concentration of illudin M in the flow through pointing out a breakthrough after 1.5 h (4.5 L). The two different trends of the linear fits are shown in red and blue. The black curve illustrates the UV signal obatined from the chromatography system