Table 1 Physiological parameters of recombinant E. coli W3110 strains
W0 | W1 | W2 | W3 | W4 | W5 | W6 | W7 | W8 | |
|---|---|---|---|---|---|---|---|---|---|
Growth rate (g cdw/L/h) | 0.067 ± 0.005 | 0.077 ± 0.001 | 0.059 ± 0.002 | 0.050 ± 0.004 | 0.051 ± 0.001 | 0.052 ± 0.002 | 0.057 ± 0.004 | 0.055 ± 0.003 | 0.059 ± 0.003 |
Final biomass (g cdw/L) | 4.84 ± 0.35 | 5.55 ± 0.06 | 4.24 ± 0.11 | 3.58 ± 0.27 | 3.65 ± 0.10 | 3.72 ± 0.15 | 4.09 ± 0.28 | 3.99 ± 0.21 | 4.25 ± 0.22 |
C t4Hyp (g/L) | 2.94 ± 0.27 | 5.90 ± 0.29 | 3.72 ± 0.27 | 1.80 ± 0.03 | 3.88 ± 0.29 | 5.60 ± 0.14 | 2.51 ± 0.06 | 7.65 ± 0.20 | 7.89 ± 0.16 |
Y t4Hyp (g t4Hyp/g cdw) | 0.61 ± 0.05 | 1.07 ± 0.06 | 0.88 ± 0.04 | 0.51 ± 0.02 | 1.06 ± 0.06 | 1.51 ± 0.09 | 0.62 ± 0.05 | 1.92 ± 0.05 | 1.86 ± 0.06 |
Y t4Hyp (g t4Hyp/g glc) | 0.07 ± 0.01 | 0.12 ± 0.01 | 0.11 ± 0.00 | 0.06 ± 0.00 | 0.13 ± 0.01 | 0.16 ± 0.00 | 0.08 ± 0.00 | 0.21 ± 0.00 | 0.22 ± 0.00 |
r t4Hyp (g t4Hyp/L/h) | 0.041 ± 0.004 | 0.082 ± 0.004 | 0.052 ± 0.004 | 0.025 ± 0.000 | 0.054 ± 0.004 | 0.078 ± 0.002 | 0.035 ± 0.001 | 0.106 ± 0.002 | 0.110 ± 0.002 |