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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Modular reconstruction and optimization of the trans-4-hydroxy-L-proline synthesis pathway in Escherichia coli

Fig. 3

Effect of vectors on P4H activity and t4Hyp production. a The specific activity of the B0-B9 strains. b The t4Hyp production of the B0 and B5 strains. (B0: BL21 (DE3), p2: pET24a, Ptrp::p4h; B1: BL21 (DE3), p5: pACYC-Duet-1, Ptrp::p4h; B2: BL21 (DE3), p6: pET24a, p4h; B3: BL21 (DE3), p7: pKK223, p4h; B4: BL21 (DE3), p8: pGEX-6P-1, p4h; B5: BL21 (DE3), p9: pMAL-C2-X, p4h; B6: BL21 (DE3), p10: pET20b, p4h; B7: BL21 (DE3), p11: pET39b, p4h; B8: BL21 (DE3), p12: pET43.1a, p4h; B9: BL21 (DE3), p13: pET48b, p4h)

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