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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Phosphite synthetic auxotrophy as an effective biocontainment strategy for the industrial chassis Pseudomonas putida

Fig. 5

PSAG-9 could not use other various P sources except Pt. A spot assay was performed on seven different types of agar-containing media: LB, Columbia, TB, Soil extract, MOPS-0 (P-free MOPS-glucose), MOPS-glucose-Pi, and MOPS-glucose-Pt. Three biological replicates were plated in different dilutions for the wild type (1, 2 and 3) and PSAG-9 (4, 5 and 6) respectively. For the spots, dilutions from an overnight culture ranging from 10–1 to 10–6 were used. Pictures were taken after 21 days of incubation at 30 °C

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Microbial Cell Factories

ISSN: 1475-2859

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