Fig. 4

Phenotypic characterization of PSAG-9. Growth in MOPS supplemented with 50 mM glucose (A), 50 mM succinate (B), and 50 mM citrate (C). D Growth in MOPS supplemented with 50 mM glucose to test different Pt sources. As P sources, Pi (triangle) was used to grow P. putida wild type, and Pt (square) was used to grow P. putida PSAG-9, employing for this purpose either phosphorous acid (yellow) or sodium phosphite (orange) as Pt source. E Comparison between the transformation efficiency in P. putida KT2440 and P. putida PSAG-9. Transformation efficiency was calculated using the expression vector pSEVAb23 pAND105 sfGFP. CFU count was performed in plates containing MOPS-glucose agar containing either 1 mM Pi (wild type) or 1 mM Pt (PSAG-9) 48 h after electrotransformation of the strains. F Growth of strains harboring the expression vector pSEVAb23 pAND105 sfGFP, in MOPS supplemented with 50 mM glucose and corresponding P sources. G Relative fluorescence of strains harboring the expression vector pSEVAb23 pAND105 sfGFP. Data represent normalized fluorescence levels, expressed as a ratio with the OD₆₀₀ over 24 h. Growth and fluorescence were monitored using a Synergy Mx Multi-Mode Microplate Reader or an Epoch 2 Microplate Spectrophotometer (BioTek Instruments, Inc., VT, U.S.). Error bars represent the standard deviation among biological duplicates and technical triplicates for each condition