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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Phosphite synthetic auxotrophy as an effective biocontainment strategy for the industrial chassis Pseudomonas putida

Fig. 1

Graphical representation of the different stages of the generation of a phosphite auxotrophic P. putida strain. A In blue, P. putida KT2440 with its native Pi-transport-related genes. Pi, Pn and G3Pi stand for phosphite, phosphonate and glycerol-3-phosphate. B In green, P. putida PSAG with its corresponding native genes, plus the hypophosphite transporter HtxBCDE from P. stutzeri WM88 and the phosphite dehydrogenase PtxD from Ralstonia sp. 4506. Pt stands for phosphite. C In yellow, P. putida PSAG-9 equipped only with the phosphite assimilation genes htxBCDE and ptxD and deprived of its native Pi-transport related genes

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