From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris

Table 1 Overview of overexpression and knockout targets tested in this work for IgG producing P. pastoris

Gene	Name description	Product description	Cellular localization	References
YAP1	Yeast activator protein 1	Transcription factor involved in the regulation of transcription in response to oxidative stress	Nucleus and cytoplasm	[26]
HAC1	Homologous to Atf/Creb1	Transcription factor involved in the regulation of the unfolded protein response	Nucleus	[27]
PDI1	Protein disulfide isomerase 1	Multifunctional protein disulfide isomerase and protein disulfide oxidoreductase	ER lumen	[8]
SBH1	Sec61 beta homolog 1	Beta subunit of Sec61 ER translocation complex	ER membrane	[28]
CPR5	Cyclosporin-sensitive proline rotamase 5	Rearranges peptidyl-prolyl bonds; transcriptionally induced in response to the UPR	ER lumen	[9]
KAR2	Karyogamy gene 2	ATPase involved in protein import into the ER, mediates protein folding, and regulates UPR	ER lumen	[6]
LHS1	Luminal Hsp70 protein 1	UPR regulated chaperone of the ER lumen involved in polypeptide translocation and folding	ER lumen	[29]
YPT7^a	Yeast protein transport 7	Rab family GTPase involved in membrane trafficking	Vacuolar, endosomal, Golgi and mitochondrial membrane	[28]

ISSN: 1475-2859