Table 4 Target genes, primers, and homologous templates used in this study
From: Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker’s yeast in bread dough
Gene | Targeted sequence | PAM | Primer sequence for colony PCR | ssDNA template sequence |
|---|---|---|---|---|
ADE2 | CCGGTTGTGGTATATTTGGTGTGGA | TTTC | F: TCTAAGTACATCCTACTATAACAATC R: GGACACTTATATGTCGAGCAAGA | TTACTTGTTTTCTAGATAAGCTTCGTAACCGACAGTTTCTAACTTTTGTGCTTTGACAAGACAATCATACGTCCCAATTGTCCCCCTCCTAATATACCAACTGTTCTAGAATCCAT |
NTH1 | CACATAGCTGAAGTTGTTATAGAAT | TTTC | F: GACAGGAGCATCGTGTAGGA R: CTACCTAGGAGCGGCCAA | CTATAGTCCATAGAGGTTTCTTTCTTGAGGCCTTAAACTGCTAAAGAATGATATTGGTGGTGATAATCTTCTTTGACGGCCTTGGGCTACCGGTCCTTGGCTTGTATTAACTTGACTCAT |
HSP12 | GGCAGAGTCGTGGACACCTTGGAAG | TTTC | F: CCCACAAACACAGACCAGAA R: CGCGGAAATTGATGACGAA | ATGTCTGACGCAGGTAGAAAAGGATTCGGTGAAAAAGCTTCTGAAGCTTTGAAGCCAGACGATGCCGTCGAATATGTTTCCGGTCGTGTCCACGGTGAAGAAGACCCAACCAAGAAGTAA |