Table 3 Characteristics of strains and plasmids used in this study
From: Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker’s yeast in bread dough
Strains or plasmids | Characteristics | Reference or source |
|---|---|---|
S. cerevisiae BCRC21447 | MATa/a industrial brewer’s top yeast | Bioresource collection and research center |
S. cerevisiae Δnth1 | MATa/a, Δnth1 + pBCoN1 | This study |
S. cerevisiae Δhsp12 | MATa/a, Δhsp12 + pBCoH1 | This study |
S. cerevisiae Δnth1/Δhsp12 | MATa/a, Δnth1Δhsp12 + pBCoNH1 | This study |
pUDC175 | E. coli/S. cerevisiae shuttle vector, containing Amp+, TRP1+, FnCpf1 | Świat et al. [23] |
pUDE710 | E. coli/S. cerevisiae shuttle vector, containing Amp+, KanMX+, crADE2-crHIS4 | Świat et al. [23] |
pBCo1 | E. coli/S. cerevisiae shuttle vector, containing Amp+, KanMX+, FnCpf1, crADE2 | This study |
pBCoN1 | E. coli/S. cerevisiae shuttle vector, containing Amp+, KanMX+, FnCpf1, crNTH1.2 | This study |
pBCoH1 | E. coli/S. cerevisiae shuttle vector, containing Amp+, KanMX+, FnCpf1, crHsp12.1 | This study |
pBCoNH1 | E. coli/S. cerevisiae shuttle vector, containing Amp+, KanMX+, FnCpf1, crNTH1.2-crHsp12.1 | This study |